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CD138/Syndecan-1 Polyclonal antibody

CD138/Syndecan-1 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 10593-1-AP

Synonyms

CD138, CD138/Syndecan-1, SDC, SDC1, SYND1, syndecan, syndecan 1



Tested Applications

Positive WB detected inA549 cells, mouse spleen tissue, HeLa cells, Raji cells
Positive IHC detected inhuman tonsillitis tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy CD138/SDC1 Ready-To-Use IHC Kit. CD138/SDC1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:800-1:3200
Sample-dependent, check data in validation data gallery

Product Information

10593-1-AP targets CD138/Syndecan-1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD138/Syndecan-1 fusion protein Ag0823 相同性解析による交差性が予測される生物種
Full Name syndecan 1
Calculated molecular weight 32.5 kDa
Observed molecular weight60-100 kDa, 130 kDa
GenBank accession numberBC008765
Gene symbol SDC1
Gene ID (NCBI) 6382
RRIDAB_2182736
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD138, also named as Syndecan-1 (SDC1), is an integral membrane protein. It participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. It is a heparan sulfate proteoglycan expressed on the surface of, and actively shed by, myeloma cells. Altered syndecan-1 expression has been detected in several different tumor types. CD138 was regarded as a useful marker for labeling normal and neoplastic plasma cells and plasmacytoid lymphomas. CD138 has a calculated molecular weight of 32.5 kDa, the apparent molecular weight is higher due to extensive glycosylation.

Protocols

Product Specific Protocols
WB protocol for CD138/Syndecan-1 antibody 10593-1-APDownload protocol
IHC protocol for CD138/Syndecan-1 antibody 10593-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cell

Tumor-reactive antibodies evolve from non-binding and autoreactive precursors.

Authors - Roei D Mazor
humanWB,IP

Autophagy

SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes

Authors - Wang Zheng
mouseIHC

Leukemia

miR-21 antagonism abrogates Th17 tumor promoting functions in multiple myeloma.

Authors - Marco Rossi
mouseIF

Theranostics

Aurka loss in CD19+ B cells promotes megakaryocytopoiesis via IL-6/STAT3 signaling-mediated thrombopoietin production.

Authors - Xin Chen

Mol Neurodegener

Amelioration of amyloid-β-induced deficits by DcR3 in an Alzheimer's disease model.

Authors - Yi-Ling Liu
humanWB

Cell Mol Life Sci

The association of telomere maintenance and TERT expression with susceptibility to human papillomavirus infection in cervical epithelium.

Authors - Li-Na Wang