RPS14 Polyclonal antibody
RPS14 Polyclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (2)
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 16683-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | HeLa cells, mouse liver tissue, HepG2 cells, rat liver tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | HepG2 cells, U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| WB | See 5 publications below |
| IHC | See 1 publications below |
| IF | See 4 publications below |
Product Information
16683-1-AP targets RPS14 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat, Arabidopsis, mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | RPS14 fusion protein Ag10012 相同性解析による交差性が予測される生物種 |
| Full Name | ribosomal protein S14 |
| Calculated molecular weight | 16 kDa |
| Observed molecular weight | 16 kDa |
| GenBank accession number | BC006784 |
| Gene symbol | RPS14 |
| Gene ID (NCBI) | 6208 |
| RRID | AB_2180158 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The mammalian ribosome consists of 4 RNA species and approximately 80 different proteins. The ribosomal proteins are encoded by complex gene families that include at least 1 active intron-containing gene and multiple processed pseudogenes[ PMID 3785212]. The RPS14 gene, encoding a ribosomal protein that belongs to the 40S ribosome, and mapping to the commonly deleted region (CDR) of the 5q- syndrome, shows haploinsufficiency in the CD34+ cells of patients with this disorder[PMID:22430631].
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RPS14 antibody 16683-1-AP | Download protocol |
| IHC protocol for RPS14 antibody 16683-1-AP | Download protocol |
| IF protocol for RPS14 antibody 16683-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nucleic Acids Res Selenite targets eIF4E-binding protein-1 to inhibit translation initiation and induce the assembly of non-canonical stress granules. | ||
J Exp Bot The in vivo functions of ARPF2 and ARRS1 in ribosomal RNA processing and ribosome biogenesis in Arabidopsis. | ||
Free Radic Biol Med Identification of interacting partners of Human Mpv17-like protein with a mitigating effect of mitochondrial dysfunction through mtDNA damage. | ||
J Exp Bot Retention of OsNMD3 in the cytoplasm disturbs protein synthesis efficiency and affects plant development in rice. | ||
Blood Adv TLR7 ligation augments hematopoiesis in Rps14 (uS11) deficiency via paradoxical suppression of inflammatory signaling. |
