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  • KD/KO Validated

PDK4 Polyclonal antibody

PDK4 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12949-1-AP

Synonyms

PDK4



Tested Applications

Positive WB detected inmouse liver tissue, NCI-H1299 cells, mouse heart tissue, mouse skeletal muscle tissue, mouse kidney tissue, rat heart tissue
Positive IHC detected inhuman lung cancer tissue, human pancreas cancer tissue, mouse heart tissue, mouse skeletal muscle tissue, rat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy PDK4 Ready-To-Use IHC Kit. PDK4 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

12949-1-AP targets PDK4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, goat, rat, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDK4 fusion protein Ag3629 相同性解析による交差性が予測される生物種
Full Name pyruvate dehydrogenase kinase, isozyme 4
Calculated molecular weight 411 aa, 46 kDa
Observed molecular weight 46 kDa
GenBank accession numberBC040239
Gene symbol PDK4
Gene ID (NCBI) 5166
RRIDAB_2161499
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Pyruvate dehydrogenase kinase isoform4 (PDK4) is also named as PDHK4 and belongs to the PDK/BCKDK protein kinase family. It is upregulated by starvation in many tissues of the body during starvation. This causes inactivation of the pyruvate dehydrogenase complex which blocks pyruvate oxidation and conserves lactate and alanine for gluconeogenesis. Enhanced PDK4 expression may be caused by the increase in free fatty acids that occurs during starvation. Free fatty acids can activate peroxisome proliferator-activated receptor α (PPARα), and activation of PPARα can promote PDK4 expression (PMID: 11554740).

Protocols

Product Specific Protocols
WB protocol for PDK4 antibody 12949-1-APDownload protocol
IHC protocol for PDK4 antibody 12949-1-APDownload protocol
IF protocol for PDK4 antibody 12949-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nat Cell Biol

Single-cell reconstruction of the adult human heart during heart failure and recovery reveals the cellular landscape underlying cardiac function.

Authors - Li Wang
humanWB

Sci Transl Med

Loss of miR-31-5p drives hematopoietic stem cell malignant transformation and restoration eliminates leukemia stem cells in mice.

Authors - Biying Zhu
humanWB

Mol Syst Biol

STAT3-dependent analysis reveals PDK4 as independent predictor of recurrence in prostate cancer.

Authors - Monika Oberhuber
humanWB

Theranostics

ITGB2-mediated metabolic switch in CAFs promotes OSCC proliferation by oxidation of NADH in mitochondrial oxidative phosphorylation system.

Authors - Xiaoxin Zhang
mouseIF

Diabetes

Glucose controls glucagon secretion by regulating fatty acid oxidation in pancreatic alpha cells

Authors - Sarah L Armour
mouseWB

PLoS Genet

PMID: 27711113

Authors - Xiaolin Lin
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Wei (Verified Customer) (01-30-2020)

Strong, sharp band as predicted size around 50kDa. It downregulates in DCM samples.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse Heart