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OXTR Polyclonal antibody

OXTR Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (3)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 23045-1-AP

Synonyms

OT R, OXTR, Oxytocin receptor



Tested Applications

Positive WB detected inJurkat cells, HeLa cells, L02 cells
Positive IP detected inL02 cells
Positive IHC detected inhuman hysteromyoma tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

23045-1-AP targets OXTR in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, rat, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen OXTR fusion protein Ag19074 相同性解析による交差性が予測される生物種
Full Name OXTR
Calculated molecular weight 389 aa, 43 kDa
Observed molecular weight 46 kDa, 67 kDa
GenBank accession numberBC137443
Gene symbol OXTR
Gene ID (NCBI) 5021
RRIDAB_2827425
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Oxytocin (OXT) is a neurohypophysial hormone that plays a role in lactation and parturition and in the central nervous system as a neurotransmitter involved in sex and maternal behavior (PMID: 1852313; 10811917). OXT acts through its receptor, OXTR, which belongs to the G protein-coupled 7-transmembrane receptor family. OXTR is expressed in endometrium, myometrium, mammary gland (PMID: 1313946). This antibody is raised against the C-terminal region of human OXTR. Two immunoreactive OXTR bands were found in the fat tissue, an unglycosylated 43 kDa form and a mature glycosylated 67 kDa form (PMID:32819381).

Protocols

Product Specific Protocols
WB protocol for OXTR antibody 23045-1-APDownload protocol
IHC protocol for OXTR antibody 23045-1-APDownload protocol
IP protocol for OXTR antibody 23045-1-APDownload protocol
FC protocol for OXTR antibody 23045-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB,IF

Nat Commun

PMID: 24915299

Authors - Christian Elabd
mouseIF

Acta Physiol (Oxf)

Oxytocin modulates neural processing of mitral/tufted cells in the olfactory bulb.

Authors - Changcheng Sun
mouseIF

Oxid Med Cell Longev

PMID: 32082478

Authors - Daniela C Wigger
mouseIHC

Antioxidants (Basel)

ΔMST and the Regulation of Cardiac CSE and OTR Expression in Trauma and Hemorrhage.

Authors - Britta Trautwein
humanWB

Biochem Pharmacol

Synergistic antitumor activity of 5-fluorouracil and atosiban against microsatellite stable colorectal cancer through restoring GATA3.

Authors - Mengmeng Wang
  • KD Validated
humanWB

Front Pharmacol

The Role of Formyl Peptide Receptor 1 in Uterine Contraction During Parturition.

Authors - Chaolu Chen