NPHP4 Polyclonal antibody

NPHP4 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, Canine

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 13812-1-AP

Synonyms

KIAA0673, Nephrocystin 4, nephronophthisis 4, Nephroretinin, NPHP4, SLSN4



Tested Applications

Positive WB detected inmouse brain tissue, Y79 cells
Positive IHC detected inhuman heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhTERT-RPE1 cells, MDCK cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

13812-1-AP targets NPHP4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, Canine samples.

Tested Reactivity human, mouse, Canine
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NPHP4 fusion protein Ag4875 相同性解析による交差性が予測される生物種
Full Name nephronophthisis 4
Calculated molecular weight 1426 aa, 158 kDa
Observed molecular weight 150 kDa
GenBank accession numberBC040520
Gene symbol NPHP4
Gene ID (NCBI) 261734
RRIDAB_10640302
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

NPHP4, also named as KIAA0673, is a cilia-associated protein which involved in the organization of apical junctions in kidney cells together with NPHP1 and RPGRIP1L/NPHP8. It is necessary for normal photoreceptor ribbon synapse maintenance and outer segment formation, and for sperm development.

Protocols

Product Specific Protocols
WB protocol for NPHP4 antibody 13812-1-APDownload protocol
IHC protocol for NPHP4 antibody 13812-1-APDownload protocol
IF protocol for NPHP4 antibody 13812-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseIF

Nat Genet

A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.

Authors - Garcia-Gonzalo Francesc R FR
mouseIF

Nat Cell Biol

Lineage specificity of primary cilia in the mouse embryo.

Authors - Fiona K Bangs
humanIF

Sci Adv

Ciliopathy protein HYLS1 coordinates the biogenesis and signaling of primary cilia by activating the ciliary lipid kinase PIPKIγ.

Authors - Chuan Chen
humanIF

Nat Commun

Microtubule asters anchored by FSD1 control axoneme assembly and ciliogenesis.

Authors - Hai-Qing Tu
mouseIF

J Cell Biol

The small GTPase RSG1 controls a final step in primary cilia initiation.

Authors - Stephanie O Agbu
humanIF

Genetics

Primary Cilium Formation and Ciliary Protein Trafficking Is Regulated by the Atypical MAP Kinase MAPK15 in Caenorhabditis elegans and Human Cells.

Authors - Anna Kazatskaya