Mouse TGF-beta1 ELISA Kit1 Publications

カタログ番号: KE10005

74,000円

(希望販売価格)

容量: 1 Kit (96 assays)

販売および在庫状況はコスモ・バイオ(株)にお問い合わせください。
distributor

Images See all

概要


製品名:
Mouse TGF-beta1 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
16.0 pg/mL

測定範囲:
31.25-2000 pg/mL

測定可能動物種:
Mouse

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 91% 80%-102%
Mouse serum 78% 68%-101%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 72.4 3.3 4.5
2 20 154.8 11.1 7.2
3 20 295.8 19.7 6.7

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 58.9 4.4 7.5
2 24 128.0 6.8 5.3
3 24 248.0 17.4 7.0

製品概要
KE10005 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TGF-beta1 ELISA kit is to be used to detect and quantify protein levels of endogenous TGF-beta1. The assay recognizes mouse TGF-beta1. A monoclonal antibody specific for TGF-beta1 has been pre-coated onto the microwells. The TGF-beta1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for TGF-beta1 is added to detect the captured TGF-beta1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
TGF beta1, Tgfb, Tgfb 1, Tgfb1, TGFbeta1, TGF-beta1, TGF-beta-1
背景説明

TGF-beta is a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. TGF-beta is produced by a number of cell types including regulatory T cells, fibroblasts, epithelial cells, and endothelial cells. TGF-beta acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. TGF-beta plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Mice lacking TGF-beta develop severe multifocal inflammatory disease, yolk sac defects and colon cancer.


Back
to top