Mouse MCP-1 ELISA Kit

Sensitivity

1.3 pg/mL

Range

3.13-200 pg/mL

Reactivity

Mouse

Cat no : KE10006

Synonyms

AI323594, Ccl2, chemokine (C C motif) ligand 2, HC11, JE, MCAF, MCP 1, Mcp1, MCP-1, Scya2, Sigje, SMC CF



Product Information

KE10006 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The MCP-1 ELISA kit is to be used to detect and quantify protein levels of endogenous MCP-1. The assay recognizes mouse MCP-1. An antibody specific for MCP-1 has been pre-coated onto the microwells. The MCP-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for MCP-1 is added to detect the captured MCP-1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Mouse MCP-1 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 1.3 pg/mL
Range 3.13-200 pg/mL
Reactivity Mouse
Tested applications Sandwich ELISA
Gene ID (NCBI) 20296

Recovery

Sample TypeAverageRange
Mouse serum91% 76%-127%
Cell culture supernatants102%85%-119%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120196.217.48.8
22052.32.95.5
32011.20.76.6

InterAssay

Samplenmean ( pg/mL)SDCV%
124110.19.28.4
22433.12.68.0
3248.50.78.4

Background Information

Monocyte chemotactic protein 1 (Mcp1; also known as Ccl2), is chemotactic for monocyte/macrophage, B-cell, and T-lymphocyte, and belongs to the CC subfamily of chemokines. Chemokines are a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The human ortholog has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis, and atherosclerosis.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms AI323594, Ccl2, chemokine (C C motif) ligand 2, HC11, JE, MCAF, MCP 1, Mcp1, MCP-1, Scya2, Sigje, SMC CF

Publications

SpeciesSample TypeTitle
mouseserum

Nat Commun

Deficiency of WTAP in hepatocytes induces lipoatrophy and non-alcoholic steatohepatitis (NASH)

Authors - Xinzhi Li
mousecell culture supernatants

ACS Appl Mater Interfaces

Prussian Blue Nanozymes Prevent Anthracycline-Induced Liver Injury by Attenuating Oxidative Stress and Regulating Inflammation.

Authors - Huiyuan Bai
mouseserum

Adv Healthc Mater

Enhanced Antibacterial And Wound Healing Using A Metal-Organic Cluster Inspired by Artificial Photosynthesis

Authors - Long Zhu
mouseserum

Int J Biol Sci

Olfactomedin-4 deletion exacerbates DSS-induced colitis through a matrix metalloproteinase-9-dependent mechanism

Authors - Xinyu Wang
mousecell culture supernatants

Cancer Lett

SIGLEC15 amplifies immunosuppressive properties of tumor-associated macrophages in pancreatic cancer.

Authors - Tian-Jiao Li
mousetissue homogenates

Stem Cell Rev Rep

Mesenchymal Stem Cell-Derived Exosomal miR-150-3p Affects Intracerebral Hemorrhage By Regulating TRAF6/NF-κB Axis, Gut Microbiota and Metabolism

Authors - Jingchi Sun