Mouse IL-17A ELISA Kit

Sensitivity

2.6 pg/mL

Range

7.8-500 pg/mL

Reactivity

Mouse

Cat no : KE10020

Synonyms

Ctla 8, Ctla8, CTLA-8, Il17, IL-17, Il17a, IL-17A, interleukin 17A, Interleukin-17A



Product Information

KE10020 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mouse IL-17A ELISA kit is to be used to detect and quantify protein levels of endogenous mouse IL-17A. The assay recognizes mouse IL-17A. An antibody specific for mouse IL-17A has been pre-coated onto the microwells. The mouse IL-17A protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for mouse IL-17A is added to detect the captured mouse IL-17A protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Mouse IL-17A ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants, Tissue lysates
Assay type Sandwich
Sensitivity 2.6 pg/mL
Range 7.8-500 pg/mL
Reactivity Mouse
Tested applications Sandwich ELISA
Gene ID (NCBI) 16171

Recovery

Sample TypeAverageRange
Mouse serum93%74%-112%
Cell culture supernatants93%87%-105%

IntraAssay

Samplenmean ( pg/mL)SDCV%
1209.90.88.0
220104.54.54.3
320386.526.16.8

InterAssay

Samplenmean ( pg/mL)SDCV%
12410.21.09.6
22493.78.69.2
324428.242.19.8

Background Information

Il17a, also named as Il-17, is a proinflammatory cytokine. Il-17, synthesized only by memory T cells and natural killer cells, has pleiotropic effects, mainly in the recruitment and activation of neutrophils. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. The Il-17 receptor is a type I transmembrane protein, that is widely expressed on epithelial cells, fibroblasts, B and T cells, and monocytic cells. In psoriatic skin lesions, both Th17 cells and their downstream effector molecules, e.g. Il-17 and IL-22, are highly increased.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms Ctla 8, Ctla8, CTLA-8, Il17, IL-17, Il17a, IL-17A, interleukin 17A, Interleukin-17A

Publications

SpeciesSample TypeTitle
mouse

Oxid Med Cell Longev

Simvastatin Reduces NETosis to Attenuate Severe Asthma by Inhibiting PAD4 Expression

Authors - Yun-Rong Chen
mousecorneal allografts

Am J Transplant

The NLRP3 inflammasome regulates corneal allograft rejection through enhanced phosphorylation of STAT3.

Authors - Chao Wei
mouseserum

J Nanobiotechnology

Green Phellodendri Chinensis Cortex-based carbon dots for ameliorating imiquimod-induced psoriasis-like inflammation in mice.

Authors - Meiling Zhang
mousecell culture supernatants

Front Immunol

IFN-γ-Primed hUCMSCs Significantly Reduced Inflammation via the Foxp3/ROR-γt/STAT3 Signaling Pathway in an Animal Model of Multiple Sclerosis.

Authors - Xiao Ling
mousetissue homogenates

Biomed Pharmacother

Aster tataricus attenuates asthma efficiently by simultaneously inhibiting tracheal ring contraction and inflammation.

Authors - Yijun Chen
mousetissue homogenates

Eur J Med Res

Research on the mechanism of prednisone in the treatment of ITP via VIP/PACAP-mediated intestinal immune dysfunction

Authors - Xiang Yan