Mouse IL1-beta ELISA Kit4 Publications

カタログ番号: KE10003

74,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Mouse IL1-beta ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant, Cell lysate

アッセイ方法:
Sandwich

感度:
1.0 pg/mL

測定範囲:
7.8-500 pg/mL

測定可能動物種:
Mouse

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 113% 89%-126%
Cell lysate 104% 82%-128%
Mouse serum 102% 91%-123%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 520.0 17.0 3.3
2 20 159.5 5.6 3.5
3 20 29.1 0.5 1.8

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 485.5 20.1 4.1
2 24 156.6 4.8 3.1
3 24 28.5 1.3 4.6

製品概要
KE10003 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL-1β ELISA kit is to be used to detect and quantify protein levels of endogenous IL-1β. The assay recognizes mouse IL-1β. A monoclonal antibody specific for IL-1β has been pre-coated onto the microwells. The IL-1β protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for IL-1β is added to detect the captured IL-1β protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.
NB: presursor and active form can be detected by this kit.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
Il 1b, IL 1beta, IL-1, Il1b, IL-1b, interleukin 1 beta
背景説明

Interleukin-1 is a pro-inflammatory cytokine with multiple biological effects. The IL-1 gene family encodes three proteins: IL-1α, IL-1β and their naturally occurring inhibitor IL-1RN. Interleukin 1β(IL-1β), mainly produced by blood monocytes and tissue macrophages, has been implicated in mediating both acute and chronic inflammation. IL-1β is known to be involved in a variety of cellular activities, including cell proliferation, differentiation and apoptosis. IL-1β is emerging as a key mediator of carcinogenesis that characterizes host-environment interactions.


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