MZB1 Polyclonal antibody

MZB1 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF

Conjugate

Unconjugated

Cat no : 11454-1-AP

Synonyms

hypothetical protein MGC29506, MZB1, PACAP, pERp1



Tested Applications

Positive WB detected inRaji cells
Positive IHC detected inhuman gliomas tissue, human small intestine tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy MZB1 Ready-To-Use IHC Kit. MZB1 primary antibody included.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

11454-1-AP targets MZB1 in WB, IHC, IF applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MZB1 fusion protein Ag2015 相同性解析による交差性が予測される生物種
Full Name hypothetical protein MGC29506
Calculated molecular weight 189 aa, 21 kDa
Observed molecular weight 19-21 kDa
GenBank accession numberBC021275
Gene symbol MGC29506
Gene ID (NCBI) 51237
RRIDAB_832820
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

MZB1, also named as ,PACAP, MGC29506 and pERp1, belongs to the PERP1 family. It is associates with immunoglobulin M (IgM) heavy and light chains and promotes IgM assembly and secretion. MGC29506(PACAP) may exert its effect by acting as a molecular chaperone or as an oxidoreductase as it displays a low level of oxidoreductase activity. Isoform 2 may be involved in regulation of apoptosis. (PubMed: 11350957) MZB1 has 5 isoforms with MW 21kd and 7-13kd.

Protocols

Product Specific Protocols
WB protocol for MZB1 antibody 11454-1-APDownload protocol
IHC protocol for MZB1 antibody 11454-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Clin Cancer Res

Integrative array-based approach identifies MZB1 as a frequently methylated putative tumor suppressor in hepatocellular carcinoma.

Authors - Matsumura Satoshi S
humanIHC,IF

J Pathol

Proteomic profiling identifies novel diagnostic biomarkers and molecular subtypes for mucinous tubular and spindle cell carcinoma of the kidney.

Authors - Huiya Xu
humanIHC

Int J Cancer

Epigenetic suppression of the immunoregulator MZB1 is associated with the malignant phenotype of gastric cancer.

Authors - Mitsuro Kanda
humanWB

J Biol Chem

Transgenic expression of microRNA-185 causes a developmental arrest of T cells by targeting multiple genes including Mzb1.

Authors - Belkaya Serkan S
humanWB,IHC,IF

Arthritis Res Ther

Increase of MZB1 in B cells in systemic lupus erythematosus: proteomic analysis of biopsied lymph nodes.

Authors - Aya Miyagawa-Hayashino
humanIHC

Oncol Lett

MZB1 expression indicates poor prognosis in estrogen receptor-positive breast cancer.

Authors - Manabu Watanabe