Human VEGF ELISA Kit1 Publications

カタログ番号: KE00085

74,000円

(希望販売価格)

容量: 1 Kit (96 assays)

販売および在庫状況はコスモ・バイオ(株)にお問い合わせください。
distributor

Images See all

概要


製品名:
Human VEGF ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
6.5 pg/mL

測定範囲:
15.6-1000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 108% 96%-118%
Human plasma 97% 81%-115%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 874.8 55.2 6.3
2 20 246.1 14.2 5.8
3 20 21.9 1.2 5.3

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 963.9 69.1 7.2
2 24 236.7 14.3 6.0
3 24 24.6 1.9 7.6

製品概要
KE00085 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The VEGF ELISA kit is to be used to detect and quantify protein levels of endogenous VEGF. The assay recognizes human VEGF. A monoclonal antibody specific for VEGF has been pre-coated onto the microwells. The VEGF protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for VEGF is added to detect the captured VEGF protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGFA, VPF
背景説明

Vascular endothelial growth factor (VEGF), is a signal protein produced by cells that stimulates vasculogenesis and angiogenesis. It is part of the system that restores the oxygen supply to tissues when blood circulation is inadequate such as in hypoxic conditions. Serum concentration of VEGF is high in bronchial asthma and diabetes mellitus. The activities of VEGF are not limited to the vascular system; VEGF plays a role in normal physiological functions such as bone formation, hematopoiesis, wound healing, and development. Disruption of this gene in mice resulted in abnormal embryonic blood vessel formation. VEGF is upregulated in many known tumors and its expression is correlated with tumor stage and progression.


Back
to top