Human TGF-beta1 ELISA Kit6 Publications

カタログ番号: KE00002

74,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human TGF-beta1 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant, Urine

アッセイ方法:
Sandwich

感度:
3.3 pg/mL

測定範囲:
15.6-1000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 105% 93%-115%
Urine 93% 86%-101%
Human plasma 100% 81%-111%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 632.6 28.2 4.5
2 20 81.2 3.9 4.8
3 20 18.5 0.9 4.9

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 659.6 62.0 9.4
2 24 83.3 4.5 5.5
3 24 15.5 0.3 2.0

製品概要
KE00002 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The TGF-β1 ELISA kit is to be used to detect and quantify protein levels of endogenous TGF-β1. The assay recognizes human TGF-β1. A polyclonal antibody specific for TGF-β1 has been pre-coated onto the microwells. The TGF-β1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for TGF-β1 is added to detect the captured TGF-β1 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
CED, DPD1, LAP, TGF ?1, TGF beta 1, TGFB, TGFB1, TGFbeta
背景説明

TGF-β is a member of the transforming growth factor beta (TGFB) family of cytokines, which are multifunctional peptides that regulate proliferation, differentiation, adhesion, migration, and other functions in many cell types. TGF-β is produced by a number of cell types including regulatory T cells, fibroblasts, epithelial cells, and endothelial cells. TGF-β acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. TGF-β plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. TGF-β appears to promote late stage progression and metastasis in some cancers.


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