Human SERPINA3 ELISA Kit0 Publications

カタログ番号: KE00037

47,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human SERPINA3 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma

アッセイ方法:
Sandwich

感度:
0.55 ng/mL

測定範囲:
1-64 ng/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human plasma 99% 82%-113%

IntraAssay:
Samplenmean (ng/mL)SDCV%
1 20 28.9 1.6 5.5
2 20 5.6 0.4 6.9
3 20 1.2 0.1 9.7

InterAssay:
Samplenmean (ng/mL)SDCV%
1 24 26.8 1.9 6.9
2 24 5.3 0.3 6.1
3 24 1.4 0.1 8.6

製品概要
KE00037 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The SERPINA3 ELISA kit is to be used to detect and quantify protein levels of endogenous SERPINA3. The assay recognizes human SERPINA3. A polyclonal antibody specific for SERPINA3 has been pre-coated onto the microwells. The SERPINA3 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for SERPINA3 is added to detect the captured SERPINA3 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
A1 Antichymotrypsin, AACT, ACT, Alpha 1 antichymotrypsin, GIG24, GIG25, Serpin A3, SERPINA3, SERPINA3,AACT
背景説明

Human SerpinA3, also known as Alpha 1-antichymotrypsin (AACT), is a member of the serine protease inhibitor class, and is a plasma alpha globulin glycoprotein which increases in the blood during the inflammatory process. SerpinA3, is also an inhibitor of neutrophil cathepsin G, mast cell chymases and pancreatic chymotrypsin. SerpinA3 is produced primarily in the liver, and is identified as an acute-phase inflammatory protein. SerpinA3 deficiency has been associated with liver disease. SerpinA3 has also been implicated in the pathology of a number of devastating human diseases including chronic obstructive pulmonary disease (COPD), Parkinson’s disease (PD), Alzheimer’s disease (AD), Stroke, Cystic Fibrosis, Cerebral Haemorrhage and Multiple System Atrophy.


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