Human GRP ELISA Kit0 Publications

カタログ番号: KE00070

93,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human GRP ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
0.03 ng/mL

測定範囲:
0.313-20 ng/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 91% 77%-103%
Human plasma 108% 86%-128%

IntraAssay:
Samplenmean (ng/mL)SDCV%
1 20 13.3 0.90 6.9
2 20 4.6 0.20 4.6
3 20 1.1 0.06 5.3

InterAssay:
Samplenmean (ng/mL)SDCV%
1 24 13.8 0.70 4.9
2 24 3.4 0.30 7.8
3 24 0.6 0.05 8.8

製品概要
KE00070 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The GRP ELISA kit is to be used to detect and quantify protein levels of endogenous GRP. The assay recognizes human GRP. A polyclonal antibody specific for GRP has been pre-coated onto the microwells. The GRP protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for GRP is added to detect the captured GRP protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
BN, gastrin releasing peptide, GRP, GRP 10, preproGRP, proGRP
背景説明

Gastrin-releasing peptide, also known as GRP, is normally formed by mucosal cells in the gastric antrum and by the D cells of the pancreatic islets, and its main function is to stimulate secretion of HCl by the gastric mucosa. HCl, in turn, inhibits gastrin formation. Its 148-amino acid preproprotein, following cleavage of a signal peptide, is further processed to produce either the 27-amino acid gastrin-releasing peptide or the 10-amino acid neuromedin C. These smaller peptides regulate numerous functions of the gastrointestinal and central nervous systems, including release of gastrointestinal hormones, smooth muscle cell contraction, and epithelial cell proliferation.These peptides are also likely to play a role in human cancers of the lung, colon, stomach, pancreas, breast, and prostate.


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