Human Mammaglobin A ELISA Kit0 Publications

カタログ番号: KE00071

93,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human Mammaglobin A ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant, Urine

アッセイ方法:
Sandwich

感度:
5 pg/mL

測定範囲:
125-8000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 104% 86%-125%
Urine 94% 85%-113%
Human plasma 103% 92%-111%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 6,124.8 545.7 8.9
2 20 1,593.5 105.5 6.6
3 20 343.8 20.1 5.8

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 5,411.6 483.5 8.9
2 24 2,572.7 133.4 5.2
3 24 485.1 22.9 4.7

製品概要
KE00071 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The Mammaglobin A ELISA kit is to be used to detect and quantify protein levels of endogenous Mammaglobin A. The assay recognizes human Mammaglobin A. A polyclonal antibody specific for Mammaglobin A has been pre-coated onto the microwells. The Mammaglobin A protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for Mammaglobin A is added to detect the captured Mammaglobin A protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
Mammaglobin 1, Mammaglobin A, MGB1, SCGB2A2, UGB2
背景説明

Mammaglobin A, also known as SCGB2A2, is a member of the secretoglobin superfamily. Mammaglobin A is expressed in normal mammary gland epithelium and is over-expressed in primary and metastatic human breast cancers, making it a specific molecular marker and a potential therapeutic target for breast cancer. Secreted Mammaglobin protein has been detected in the sera of some breast cancer patients and not in sera of patients with other tumor types, indicating Mammaglobin as a serum marker for breast cancer


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