Human MMP-13 ELISA Kit0 Publications

カタログ番号: KE00078

74,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human MMP-13 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
1.2 pg/mL

測定範囲:
93.8-6000 pg/ml

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 86% 79%-92%
Human plasma 90% 82%-100%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 928.6 69.5 7.5
2 20 1,630.0 101.7 6.2
3 20 3,808.3 227.1 6.0

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 1,002.6 78.1 7.8
2 24 1,833.0 111.3 6.1
3 24 4,422.9 363.0 8.2

製品概要
KE00078 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The MMP-13 ELISA kit is to be used to detect and quantify protein levels of endogenous MMP-13. The assay recognizes human MMP-13. A monoclonal antibody specific for MMP-13 has been pre-coated onto the microwells. The MMP-13 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for MMP-13 is added to detect the captured MMP-13 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
CLG3, Collagenase 3, Matrix metalloproteinase 13, MMP 13, MMP13
背景説明

MMP-13 (Matrix metalloproteinase 13), also known as collagenase-3, cleaves type I collagen and was previously thought to be the rodent analogue of human interstitial collagenase (MMP-1). MMP-3 is also known to activate several other MMPs such as MMP-7 and MMP-9. All the above make it a crucial factor in connective tissue remodeling. MMP-13 is found in hypertrophic and calcifying cartilage of mammalian growth plate. It can be detected 3 bands that the glycosylated pro MMP-13 (70 kDa), unglycosylated proform (55 kDa), and active MMP-13 enzyme (40kDa) by western blot.


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