Human JNK ELISA Kit0 Publications

カタログ番号: KE00072

93,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human JNK ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Cell lysate

アッセイ方法:
Sandwich

感度:
0.1 ng/mL

測定範囲:
1.56-100 ng/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell lysate 100% 85%-110%

IntraAssay:
Samplenmean (ng/mL)SDCV%
1 20 82.0 5.5 6.7
2 20 28.5 0.8 2.8
3 20 6.3 0.3 5.3

InterAssay:
Samplenmean (ng/mL)SDCV%
1 24 86.0 6.3 7.3
2 24 28.0 2.0 7.2
3 24 5.5 0.5 8.9

製品概要
KE00072 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The JNK ELISA kit is designed for the quantitative determination of JNK in samples of human and mouse origin. A polyclonal antibody specific for JNK has been pre-coated onto the microwells. The JNK protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for JNK is added to detect the captured JNK protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
c Jun N terminal kinase 1, JNK, JNK 46, JNK1, JNK1A2, JNK21B1/2, JNK-phospho-Thr183/Tyr185, JNK-phospho-Tyr185, MAP kinase 8, MAPK 8, MAPK8, PRKM8, SAPK1
背景説明

JNK is also named as MAPK8 (Mitogen-activated protein kinase 8), PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. JNK is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. JNK functions to phosphorylate c-Jun at N-terminal serine regulatory sites of Ser-63 and Ser-73, mapping within the transactivation domain. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. JNK activity is abnormally elevated in obesity. Furthermore, an absence of JNK results in decreased adiposity, significantly improved insulin sensitivity, and enhanced insulin receptor signaling capacity in 2 different models of mouse obesity, including ob/ob. JNK is a crucial mediator of obesity and insulin resistance and a potential target for therapeutics. The ELISA kit is suitable for testing cell lysates.


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