Human INS ELISA Kit0 Publications

カタログ番号: KE00045

74,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human INS ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma

アッセイ方法:
Sandwich

感度:
1.3 pmol/L

測定範囲:
7.8 - 500 pmol/L

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human plasma 100% 82%-123%

IntraAssay:
Samplenmean (pmol/L)SDCV%
1 20 295.7 27.7 9.4
2 20 126.0 12.0 9.5
3 20 64.6 6.2 9.6

InterAssay:
Samplenmean (pmol/L)SDCV%
1 24 314.2 24.5 7.8
2 24 149.2 13.2 8.8
3 24 76.0 7.3 9.6

製品概要
KE00045 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The INS ELISA kit is to be used to detect and quantify protein levels of endogenous INS. The assay recognizes human INS. A monoclonal antibody specific for INS has been pre-coated onto the microwells. The INS protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for INS is added to detect the captured INS protein. For signal development, Sterptravidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
ILPR, INS, insulin, IRDN
背景説明

Insulin is a peptide hormone, produced by beta cells of the pancreas, and is central to regulating carbohydrate and fat metabolism in the body. It participates in glucose utilization, protein synthesis and in the formation and storage of neutral lipids. Insulin is synthesized as a precursor molecule, proinsulin, which is processed prior to secretion. A- and B-peptides are joined together by a disulfide bond to form insulin, while the central portion of the precursor molecule is cleaved and released as the C-peptide. Defects in insulin results in type 1 diabetes mellitus.


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