Human IL8 ELISA Kit6 Publications

カタログ番号: KE00006

74,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human IL8 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
1.0 pg/ml

測定範囲:
15.6-1000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 101% 87-114%
Human plamsa 100% 80%-127%

IntraAssay:
Samplenmean (pg/ml)SDCV%
1 20 127.3 5.3 4.1
2 20 411.6 23.7 5.8
3 20 804.4 44.7 5.6

InterAssay:
Samplenmean (pg/ml)SDCV%
1 24 123.7 4.8 3.9
2 24 420.9 17.1 4.1
3 24 788.9 35.8 4.5

製品概要
KE00006 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL8 ELISA kit is to be used to detect and quantify protein levels of endogenous IL8. The assay recognizes human IL8. A polyclonal antibody specific for IL8 has been pre-coated onto the microwells. The IL8 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for IL8 is added to detect the captured IL8 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
C X C motif chemokine 8, CXCL8, CXCL8,IL8, GCP1, IL 8, IL8, IL-8, interleukin 8, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP 1, NAP1, Neutrophil activating factor, T cell chemotactic factor
背景説明

Interleukin 8 (IL8), also known as CXCL8, which is a member of the CXC chemokine family. This chemokine is secreted by a variety of cell types including monocyte/macrophages, T cells, neutrophils, fibroblasts, endothelial cells, and various tumor cell lines in response to inflammatory stimuli. IL8 has two primary functions. It induces chemotaxis in target cells, primarily neutrophils but also other granulocytes, causing them to migrate toward the site of infection. IL8 also induces phagocytosis once they have arrived. This gene is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. IL8 is also known to be a potent promoter of angiogenesis. IL8 has been associated with tumor angiogenesis, metastasis, and poor prognosis in breast cancer. IL-8 may present a novel therapeutic target for estrogen driven breast carcinogenesis and tumor progression.


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