Human IL4 ELISA Kit0 Publications

カタログ番号: KE00016

74,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human IL4 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
2.2 pg/mL

測定範囲:
15.6-1000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 95% 83%-105%
Human plamsa 103% 86%-116%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 777.0 45.1 5.8
2 20 225.5 6.4 2.8
3 20 43.3 2.4 5.5

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 703.8 33.8 4.8
2 24 212.2 13.3 6.2
3 24 42.4 1.9 4.5

製品概要
KE00016 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL4 ELISA kit is to be used to detect and quantify protein levels of endogenous IL4. The assay recognizes human IL4. A polyclonal antibody specific for IL4 has been pre-coated onto the microwells. The IL4 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for IL4 is added to detect the captured IL4 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
B cell stimulatory factor 1, Binetrakin, BSF 1, BSF1, IL 4, IL4, IL-4, interleukin 4, Pitrakinra
背景説明

Interleukin-4 (IL-4), a member of the α-helical cytokine family, is produced by activated CD4 + T cells, basophils, and mast cells. It promotes the proliferation and differentiation of antigen presenting cells. IL4 also plays a pivotal role in antibody isotype switching and stimulates the production of IgE. This cytokine has been applied in the treatment of autoimmune disorder like multiple myeloma, cancer, psoriasis, and arthritis. IL4 has also been extensively applied to inhibit detrimental effect of Th1. It may promote the growth of epithelial tumors by mediating increased proliferation and survival.


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