Human IL28A ELISA Kit0 Publications

カタログ番号: KE00011

47,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human IL28A ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant, Urine

アッセイ方法:
Sandwich

感度:
1.0 pg/mL

測定範囲:
7.8-500 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 101% 88%-109%
Urine 100% 89%-109%
Human plamsa 95% 87%-102%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 357.5 34.2 9.6
2 20 89.6 5.1 5.7
3 20 23.2 0.9 4.0

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 375.8 22.8 6.1
2 24 95.1 4.4 4.6
3 24 23.1 1.0 4.4

製品概要
KE00011 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL28A ELISA kit is to be used to detect and quantify protein levels of endogenous IL28A. The assay recognizes human IL28A. A polyclonal antibody specific for IL28A has been pre-coated onto the microwells. The IL28A protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for IL28A is added to detect the captured IL28A protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
Cytokine Zcyto20, IFN lambda 2, IFNL2, IL 28A, IL-28, IL28A, IL-28A, Interferon lambda 2, Interleukin 28A, ZCYTO20
背景説明

IL28A, also known as IFNL2, is a cytokine distantly related to type I interferons and the IL-10 family. IL-28A, IL-28B and IL-29 are three closely related cytokines classified as type III IFNs, which share many of the biological effects of type I IFNs but may have fewer side effects due to a more selective receptor distribution. All three cytokines have been shown to interact with a heterodimeric class II cytokine receptor that consists of interleukin 10 receptor, beta (IL10RB) and interleukin 28 receptor, alpha (IL28RA). IL28A is believed to play a significant role in the antiviral immune defense in the intestinal epithelium.


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