Human IL2 ELISA Kit0 Publications

カタログ番号: KE00017

74,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human IL2 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
3.6 pg/mL

測定範囲:
15.6-1000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 112% 103%-121%
Human plamsa 92% 81%-107%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 1109.7 35.8 3.2
2 20 339.3 15.7 4.6
3 20 70.8 2.0 2.9

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 1370.3 126.4 9.2
2 24 420.6 32.5 7.7
3 24 79.9 6.5 8.1

製品概要
KE00017 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL2 ELISA kit is to be used to detect and quantify protein levels of endogenous IL2. The assay recognizes human IL2. A monoclonal antibody specific for IL2 has been pre-coated onto the microwells. The IL2 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for IL2 is added to detect the captured IL2 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm and 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
Aldesleukin, IL 2, IL2, IL-2, interleukin 2, lymphokine, T cell growth factor, TCGF
背景説明

Interleukin-2 (IL-2) is a four-helix bundle, type I cytokine that functions as a growth factor for a wide range of leukocytes. In the immune system, IL-2 is essential for immune homeostasis, normal T regulatory cell function, and self-tolerance. It regulates immune cell homeostasis and has been used to treat a range of disorders including cancer and autoimmune disease. IL-2 signals through heterodimerization of theIL-2Rβand IL-2Rγreceptor subunits.


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