Human IL12 p70 ELISA Kit1 Publications

カタログ番号: KE00019

74,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human IL12 p70 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant, Urine

アッセイ方法:
Sandwich

感度:
1.5 pg/mL

測定範囲:
31.25-2000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 102% 96%-112%
Urine 81% 69%-89%
Human plamsa 105% 91%-124%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 55.9 4.9 8.8
2 20 171.2 15.3 9.0
3 20 443.8 42.5 9.6

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 98.4 8.7 8.8
2 24 394.6 37.0 9.4
3 24 1441.0 139.8 9.7

製品概要
KE00019 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IL12 p70 ELISA kit is to be used to detect and quantify protein levels of endogenous IL12 p70. The assay recognizes human IL12 p70. A monoclonal antibody specific for IL12 p70 has been pre-coated onto the microwells. The IL12 p70 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody of biotinylated specific for IL12 p70 is added to detect the captured IL12 p70 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm with the correction wavelength set at 630 nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
CLMF, CLMF p35, IL 12 subunit p35, IL 12A, IL-12, IL12A, Interleukin 12 subunit alpha, NFSK, NKSF1, P35
背景説明

Interleukin-12 (IL-12), is a cytokine that is secreted by activated phagocytes and dendritic cells and that induces interferon-γproduction by natural-killer and T lymphocytes. IL-12 (referred to as 'p70') is a 70 kDa heterodimer composed of a 35 kDa subunit (IL-12A p35) and a 40 kDa subunit (IL-12B p40). IL-12 is involved in the differentiation of native T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels. IL-12 has been shown to play a critical role in the pathogenesis of a variety of immune-related diseases.


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