Human IFNA1 ELISA Kit0 Publications

カタログ番号: KE00044

47,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human IFNA1 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma

アッセイ方法:
Sandwich

感度:
1.5 pg/mL

測定範囲:
15.6-1000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human plasma 102% 78%-121%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 114.9 9.9 8.6
2 20 348.9 19.1 5.5
3 20 688.8 64.3 9.3

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 122.4 4.7 3.9
2 24 380.4 21.6 5.7
3 24 703.5 37.3 5.3

製品概要
KE00044 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The IFNA1 ELISA kit is to be used to detect and quantify protein levels of endogenous IFNA1. The assay recognizes human IFNA1. A polyclonal antibody specific for IFNA1 has been pre-coated onto the microwells. The IFNA1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for IFNA1 is added to detect the captured IFNA1 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
IFL, IFN, IFN ALPHA, IFN alpha 1/13, IFNA@, IFNA1, IFNA13, Interferon alpha 1/13, Interferon alpha D, interferon, alpha 1, LeIF D
背景説明

IFNA1 is a member of the Type I IFN (1) family best known for their antiviral activity. It is a key cytokine regulating the activity of B cells, T-helper cells (Th cells), cDCs and natural killer cells (NK cells). Interferon-alpha induces B cell maturation into plasma cells and immunoglobulin production. Interferon-alpha plays an important role in the pathogenesis of systemic lupus erythematosus (SLE). Interferon-alpha was the first cytokine to show clinical benefit in the treatment of certain types of cancer, including melanoma, chronic myelogenous leukemia, and renal cancer.


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