Human ICAM-1 ELISA Kit

Sensitivity

11.1 pg/mL

Range

46.88-3000 pg/mL

Reactivity

Human

Cat no : KE00061

Synonyms

BB2, CD54, ICAM 1, ICAM1, ICAM-1, P3.58



Product Information

KE00061 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The ICAM-1 ELISA kit is to be used to detect and quantify protein levels of endogenous ICAM-1. The assay recognizes human ICAM-1. An antibody specific for ICAM-1 has been pre-coated onto the microwells. The ICAM-1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, another antibody of biotinylated specific for ICAM-1 is added to detect the captured ICAM-1 protein. For signal development, Streptavidin-HRP is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450 nm with the correction wavelength set at 630 nm.

Product name Human ICAM-1 ELISA Kit
Tests 1 X 96 well plate
Sample type Serum, Plasma, Cell culture supernatants
Assay type Sandwich
Sensitivity 11.1 pg/mL
Range 46.88-3000 pg/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 3383

Recovery

Sample TypeAverageRange
Human plasma102%75%-126%
Cell culture supernatants94%86%-120%

IntraAssay

Samplenmean ( pg/mL)SDCV%
120166.86.64.0
220656.718.92.9
3202,420.790.73.7

InterAssay

Samplenmean ( pg/mL)SDCV%
124172.211.96.9
224666.034.85.2
3242,522.8154.46.1

Background Information

Intercellular adhesion molecule 1 (ICAM-1, also known as CD54) is a transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues. ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin. It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum. Soluble form of ICAM-1 (sICAM-1) has been measured in various body fluids, with increased levels being found in serum from patients with cardiovascular disease, cancer and autoimmune diseases.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms BB2, CD54, ICAM 1, ICAM1, ICAM-1, P3.58