Human HDAC1 ELISA Kit0 Publications

カタログ番号: KE00033

93,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human HDAC1 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma

アッセイ方法:
Sandwich

感度:
11.0 pg/mL

測定範囲:
62.5-4000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Human plasma 100% 86%-127%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 2,406.1 197.3 8.2
2 20 499.5 19.9 4.0
3 20 163.5 6.3 3.9

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 2,282.9 158.9 7.0
2 24 484.8 24.5 5.1
3 24 157.3 6.9 4.4

製品概要
KE00033 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The HDAC1 ELISA kit is to be used to detect and quantify protein levels of endogenous HDAC1. The assay recognizes human HDAC1. A polyclonal antibody specific for HDAC1 has been pre-coated onto the microwells. The HDAC1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for HDAC1 is added to detect the captured HDAC1 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
GON 10, HD1, HDAC1, HDAC1-specific, histone deacetylase 1, RPD3, RPD3L1
背景説明

Histone deacetylases (HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues leading to the formation of a condensed and transcriptionally silenced chromatin. The protein encoded by this gene belongs to the histone deacetylase/ acuc/ apha family and is a component of the histone deacetylase complex, which is responsible for gene expression silencing. It also plays an important role in the control of cell proliferation and differentiation by interacting with RB, p53 and other transcription factors. HADC inhibition causes apoptosis in tumor cells and HDAC inhibitors may be developed as anti-cancer agents. This kit is used to quantify HDAC1 level in vivo.


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