Human GMFB ELISA Kit0 Publications

カタログ番号: KE00001

74,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human GMFB ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Cell culture supernatant

アッセイ方法:
Sandwich

感度:
23.8 pg/mL

測定範囲:
125-8000 pg/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Cell culture supernatant 91% 83%-99%
Human plamsa 87% 75%-102%

IntraAssay:
Samplenmean (pg/mL)SDCV%
1 20 6,361.0 413.5 6.5
2 20 2,641.6 143.3 5.4
3 20 1,467.3 102.0 7.0

InterAssay:
Samplenmean (pg/mL)SDCV%
1 24 5,734.9 753.2 13.1
2 24 2,553.0 164.0 6.4
3 24 1,509.7 83.0 5.5

製品概要
Human GMFB Kit is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The GMFB ELISA kit is to be used to detect and quantify protein levels of endogenous GMFB. The assay recognizes human GMFB. A monoclonal antibody specific for GMFB has been pre-coated onto the microwells. The GMFB protein in samples is captured by the coated antibody after incubation. Following extensive washing, a polyclonal antibody specific for GMFB is added to detect the captured GMFB protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-rabbit antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
Glia maturation factor beta, glia maturation factor, beta, GMF, GMF beta, GMFB
背景説明

GMFB, Glia maturation factor beta, is a 17kd protein which initially identified as a nerve growth factor implicated in nervous system development. GMF-beta protein is specific to the brain where it is expressed in glial cells (mainly astrocytes) and insome neurons. GMFB causes differentiation of brain cells, stimulation of neural regeneration, and inhibition of proliferation of tumor cells. GMFB can be considered as a prognostic predictor for SOC(Serous ovarian carcinoma) patients. This kit is used to quantify GMFB level in vivo.


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