Human FGF12 ELISA Kit
Sensitivity
0.015 ng/mL
Range
0.5-32 ng/mL
Reactivity
Human
Cat no : KE00036
Synonyms
FGF 12, FGF12, FGF12B, FHF 1, FHF1, fibroblast growth factor 12, Myocyte activating factor
Validation Data Gallery
Product Information
KE00036 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The FGF12 ELISA kit is to be used to detect and quantify protein levels of endogenous FGF12. The assay recognizes human FGF12. A polyclonal antibody specific for FGF12 has been pre-coated onto the microwells. The FGF12 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for FGF12 is added to detect the captured FGF12 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.
| Product name | Human FGF12 ELISA Kit |
| Tests | 1 X 96 well plate |
| Sample type | Plasma, Serum, Cell culture supernatants |
| Assay type | Sandwich |
| Sensitivity | 0.015 ng/mL |
| Range | 0.5-32 ng/mL |
| Reactivity | Human |
| Tested applications | Sandwich ELISA |
| Gene ID (NCBI) | 2257 |
Recovery
| Sample Type | Average | Range |
|---|---|---|
| Plasma | 91% | 72%-125% |
| Cell culture supernatant | 102% | 82%-120% |
IntraAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | ? ug/ml | ? | ?% |
| 2 | 20 | ? ug/ml | ? | ?% |
| 3 | 20 | ? ug/ml | ? | ?% |
InterAssay
| Sample | n | mean ( ng/mL) | SD | CV% |
|---|---|---|---|---|
| 1 | 20 | ? ug/ml | ? | ?% |
| 2 | 20 | ? ug/ml | ? | ?% |
| 3 | 20 | ? ug/ml | ? | ?% |
Background Information
FGF12 is a member of the fibroblast growth factor (FGF) family. FGF family members play important roles in embryogenesis, angiogenesis, and wound repair. FGF12 lacks the N-terminal signal sequence present in most of the FGF family members, but it contains clusters of basic residues that have been demonstrated to act as a nuclear localization signal. When transfected into mammalian cells, this protein accumulated in the nucleus, but was not secreted. FGF12 plays an intracellular role in the inhibition of radiation-induced apoptosis. FGF12 is expressed abundantly in developing and adult nervous systems; therefore, FGF12 was thought to be related to nervous system development and function.
Properties
| Storage Instructions | All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions. |
| Synonyms | FGF 12, FGF12, FGF12B, FHF 1, FHF1, fibroblast growth factor 12, Myocyte activating factor |