Human CD20 ELISA Kit

Sensitivity

0.33 ng/mL

Range

0.78-50 ng/mL

Reactivity

Human

Cat no : KE00082

Synonyms

B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7



Product Information

KE00082 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The CD20 ELISA kit is to be used to detect and quantify protein levels of endogenous CD20. The assay recognizes human CD20. A polyclonal antibody specific for CD20 has been pre-coated onto the microwells. The CD20 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for CD20 is added to detect the captured CD20 protein. For signal development, horseradish peroxidase (HRP)-conjugated Anti-mouse antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

Product name Human CD20 ELISA Kit
Tests 1 X 96 well plate
Sample type Cell culture supernatant, Cell lysate
Assay type Sandwich
Sensitivity 0.33 ng/mL
Range 0.78-50 ng/mL
Reactivity Human
Tested applications Sandwich ELISA
Gene ID (NCBI) 931

Recovery

Sample TypeAverageRange
Cell culture supernatant98%83%-122%
Cell lysate101%75%-121%

IntraAssay

Samplenmean ( ng/mL)SDCV%
120? ug/ml??%
220? ug/ml??%
320? ug/ml??%

InterAssay

Samplenmean ( ng/mL)SDCV%
120? ug/ml??%
220? ug/ml??%
320? ug/ml??%

Background Information

CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells.

Properties

Storage Instructions All the reagents are stored at 2-8℃ for 6 months or -20℃ for 12 months. Refer to the protocol for further storage instructions.
Synonyms B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7