Human APOL1 ELISA Kit1 Publications

カタログ番号: KE00047

93,000円

(希望販売価格)

容量: 1 Kit (96 assays)

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概要


製品名:
Human APOL1 ELISA Kit

試験方法:
1 X 96 well plate

測定サンプル対象:
Serum, Plasma, Urine

アッセイ方法:
Sandwich

感度:
0.07 ng/mL

測定範囲:
0.156-10 ng/mL

測定可能動物種:
Human

適用アプリケーション:
Sandwich ELISA

回収率:
Sample TypeAverageRange
Urine 105% 83%-127%
Human plasma 93% 85%-107%

IntraAssay:
Samplenmean (ng/mL)SDCV%
1 20 8.31 0.312 3.8
2 20 2.21 0.095 4.3
3 20 0.63 0.013 2.1

InterAssay:
Samplenmean (ng/mL)SDCV%
1 24 9.12 0.387 4.2
2 24 2.31 0.073 3.2
3 24 0.62 0.023 3.6

製品概要
KE00047 is a solid phase sandwich Enzyme Linked-Immuno-Sorbent Assay (Sandwich ELISA). The APOL1 ELISA kit is to be used to detect and quantify protein levels of endogenous APOL1. The assay recognizes human APOL1. A polyclonal antibody specific for APOL1 has been pre-coated onto the microwells. The APOL1 protein in samples is captured by the coated antibody after incubation. Following extensive washing, a monoclonal antibody specific for APOL1 is added to detect the captured APOL1 protein. For signal development, horseradish peroxidase (HRP)-conjugated antibody is added, followed by Tetramethyl-benzidine (TMB) reagent. Solution containing sulfuric acid is used to stop color development and the color intensity which is proportional to the quantity of bound protein is measurable at 450nm.

製品特性


保存方法:
All the reagents are stored at 2-8℃. Refer to the protocol for further storage instructions.

別名:
APOL, APOL1, Apolipoprotein L, Apolipoprotein L1
背景説明

Human apolipo-protein L1 (APOL1) is a minor component of plasma high density lipoprotein (HDL) particles. The human ApoL protein family was thought to be predominantly involved in lipid transport and metabolism. APOL1 is also involved in host innate immunity against Trypanosoma parasites. Once activated, APOL1 can lyse the parasite and protect human from infection. Genetic variants in APOL1 gene, which are found in African ancestry with high frequency, associate with chronic kidney disease, like focal segmental glomerulosclerosis (FSGS), HIV-associated nephropathy (HIVAN), and hypertensive nephropathy. This kit is used to quantify APOL1 level.


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