Histone-H3 Polyclonal antibody

Histone-H3 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 17168-1-AP

Synonyms

H3/n, H3/o, H3F2, H3FM, HIST2H3A, HIST2H3C, HIST2H3D, histone, histone 3, histone cluster 2, H3a, Histone H3, Histone H3.2, Histone H3/m, Histone H3/o, Histone-H3


"Histone-H3 Antibodies" Comparison

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Applications:
  • WB analysis using 17168-1-AP

    WB analysis using 17168-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17168-1-AP (Histone-H3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • WB analysis using 17168-1-AP

    WB analysis using 17168-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17168-1-AP (Histone-H3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse skeletal muscle using 17168-1-AP

    WB analysis of mouse skeletal muscle using 17168-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17168-1-AP (Histone-H3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse liver using 17168-1-AP

    WB analysis of mouse liver using 17168-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 17168-1-AP (Histone-H3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • WB analysis of mouse brain using 17168-1-AP

    WB analysis of mouse brain using 17168-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17168-1-AP (Histone-H3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IP experiment of MCF-7 using 17168-1-AP

    IP experiment of MCF-7 using 17168-1-AP

    IP result of anti-Histone-H3(IP:17168-1-AP, 4ug; Detection:17168-1-AP 1:8000) with MCF-7 cells lysate 2120 ug.

  • IHC staining of human oesophagus cancer using 17168-1-AP

    IHC staining of human oesophagus cancer using 17168-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 17168-1-AP (Histone-H3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Histone-H3 antibody included
  • IHC staining of human oesophagus cancer using 17168-1-AP

    IHC staining of human oesophagus cancer using 17168-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 17168-1-AP (Histone-H3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Histone-H3 antibody included
  • IHC staining of human skin cancer using 17168-1-AP

    IHC staining of human skin cancer using 17168-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17168-1-AP (Histone-H3 antibody) at dilution of 1:200 (under 40x lens).

    Ready-to-use IHC kits available with Histone-H3 antibody included
  • IHC staining of human skin cancer using 17168-1-AP

    IHC staining of human skin cancer using 17168-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 17168-1-AP (Histone-H3 antibody) at dilution of 1:200 (under 10x lens).

    Ready-to-use IHC kits available with Histone-H3 antibody included
  • IHC staining of human breast cancer using 17168-1-AP

    IHC staining of human breast cancer using 17168-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17168-1-AP (Histone-H3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Histone-H3 antibody included
  • IHC staining of human breast cancer using 17168-1-AP

    IHC staining of human breast cancer using 17168-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17168-1-AP (Histone-H3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with Histone-H3 antibody included
  • IF Staining of HeLa using 17168-1-AP

    IF Staining of HeLa using 17168-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Histone-H3 antibody (17168-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 17168-1-AP

    IF Staining of HeLa using 17168-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Histone-H3 antibody (17168-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  • IF Staining of HeLa using 17168-1-AP

    IF Staining of HeLa using 17168-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using Histone-H3 antibody (17168-1-AP) at dilution of 1:1200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Beta Actin antibody (66009-1-Ig, Clone: 2D4H5, red).

  • IF Staining of HeLa using 17168-1-AP

    IF Staining of HeLa using 17168-1-AP

    Immunofluorescent analysis of HeLa cells using 17168-1-AP (Histone-H3 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

  • FC experiment of HeLa using 17168-1-AP

    FC experiment of HeLa using 17168-1-AP

    1X10^6 HeLa cells were stained with 0.20ug Histone-H3 antibody (17168-1-AP, red) and control antibody (blue). Fixed with 90% MeOH.


Tested Applications

Positive WB detected inHEK-293 cells, A549 cells, mouse skeletal muscle tissue, mouse liver tissue, mouse brain tissue, Hela cells, HepG cells, MCF-7 cells, NIH/3T3 cells, mouse kidney tissue, rat kidney tissue
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman oesophagus cancer tissue, human skin cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy Histone-H3 Ready-To-Use IHC Kit. Histone-H3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:600-1:2400
Flow Cytometry (FC)FC : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

17168-1-AP targets Histone-H3 in WB, IP, IHC, IF, FC, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, goat, chicken, rat, Arabidopsis, yellow catfish, mouse, monkey, fish, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Histone-H3 fusion protein Ag10644 相同性解析による交差性が予測される生物種
Full Name histone cluster 2, H3a
Calculated molecular weight 136 aa, 15 kDa
Observed molecular weight 15-17 kDa
GenBank accession numberBC015544
Gene symbol HIST2H3A
Gene ID (NCBI) 333932
RRIDAB_2716755
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Histone-H3, histone cluster 2, H3a is the core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machinery which requires DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Histone-H3 is expressed during S phase; then expression strongly decreases as cell division slows down during the process of differentiation.

Protocols

Product Specific Protocols
WB protocol for Histone-H3 antibody 17168-1-APDownload protocol
IHC protocol for Histone-H3 antibody 17168-1-APDownload protocol
IF protocol for Histone-H3 antibody 17168-1-APDownload protocol
IP protocol for Histone-H3 antibody 17168-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Nature

A phase-separated nuclear GBPL circuit controls immunity in plants.

Authors - Shuai Huang
humanWB

Nature

The molecular basis of tight nuclear tethering and inactivation of cGAS.

Authors - Baoyu Zhao
humanWB

Signal Transduct Target Ther

TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion

Authors - Wenwen Zheng
humanWB

Cell

Targeting Epigenetic Crosstalk as a Therapeutic Strategy for EZH2-Aberrant Solid Tumors.

Authors - Xun Huang
mouseWB

Adv Mater

A Physiologically Responsive Nanocomposite Hydrogel for Treatment of Head and Neck Squamous Cell Carcinoma via Proteolysis-targeting Chimeras Enhanced Immunotherapy

Authors - Yaping Wu
humanWB

Nat Genet

N6-Methyladenosine co-transcriptionally directs the demethylation of histone H3K9me2.

Authors - Yuan Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Mi (Verified Customer) (06-25-2023)

It works very well in nuclear extracts from human brown adipocyte cells.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: human brown adipocytes
FH

Veda (Verified Customer) (04-29-2022)

IF staining test in cryo sections of skin. It does not seem to work. Maybe it only stains the nuclear membrane.

  • Applications: Immunofluorescence
FH

Pradeep (Verified Customer) (09-19-2020)

Good antibody for Western blot. Selvaraju, V., Thirunavukkarasu, M., Joshi, M. et al. Deletion of newly described pro-survival molecule Pellino-1 increases oxidative stress, downregulates cIAP2/NF-κB cell survival pathway, reduces angiogenic response, and thereby aggravates tissue function in mouse ischemic models. Basic Res Cardiol 115, 45 (2020). https://doi.org/10.1007/s00395-020-0804-4

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse Heart Tissue
FH

Tanusree (Verified Customer) (12-03-2019)

The antibody works great in Western blotting analysis.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain
FH

Ruiting (Verified Customer) (11-11-2019)

I use the Proteintech antibodies almost every week, the antibodies never failed to me. Very gald to use their products with a great price.Ruiting

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: 1:1,000
  • Cell Tissue Type: Mouse testis and brains
FH

Robert (Verified Customer) (07-18-2019)

The Histon H3 antibody gives a signal in the three nuclear samples, but not in the cytoplasmic samples.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: ST cells (porcine)
Histone-H3 Antibody Western Blot, validation (1/1000 dilution) in ST cells (porcine) (Cat no:17168-1-AP)