HMOX2 Polyclonal antibody

HMOX2 Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14817-1-AP

Synonyms

heme oxygenase (decycling) 2, Heme oxygenase 2, HMOX2, HO 2, HO2



Tested Applications

Positive WB detected inHepG2 cells, K-562 cells, mouse spleen tissue, Raji cells
Positive IP detected inRaji cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

14817-1-AP targets HMOX2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen HMOX2 fusion protein Ag6575 相同性解析による交差性が予測される生物種
Full Name heme oxygenase (decycling) 2
Calculated molecular weight 36 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC002396
Gene symbol HMOX2
Gene ID (NCBI) 3163
RRIDAB_2118843
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Heme oxygenase is the rate-limiting enzyme in the heme degradative pathway. There are 2 isozymic forms of heme oxygenase, an inducible heme oxygenase-1 (HMOX1) and a constitutive heme oxygenase-2 (HMOX2). Down-regulation of HMOX2 is associated with the increased expression of HMOX1 in human cell lines. HMOX2 is also named as HO-2 and could be implicated in the production of carbon monoxide in brain where it could act as a neurotransmitter.

Protocols

Product Specific Protocols
WB protocol for HMOX2 antibody 14817-1-APDownload protocol
IHC protocol for HMOX2 antibody 14817-1-APDownload protocol
IF protocol for HMOX2 antibody 14817-1-APDownload protocol
IP protocol for HMOX2 antibody 14817-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Mol Cell

Metabolic-scale gene activation screens identify SLCO2B1 as a heme transporter that enhances cellular iron availability.

Authors - Gokhan Unlu
ratWB

Acta Pharm Sin B

Organic carbon monoxide prodrug, BW-CO-111, in protection against chemically-induced gastric mucosal damage.

Authors - Dominik Bakalarz
humanWB

J Cell Sci

Role of epithelial sodium channels (ENaCs) in endothelial function.

Authors - Dongqing Guo
human,mouseWB,IHC

Oxid Med Cell Longev

Hydrogen Sulfide Abrogates Hemoglobin-Lipid Interaction in Atherosclerotic Lesion.

Authors - László Potor
ratWB

Cells

Evidence for Cytoprotective Effect of Carbon Monoxide Donor in the Development of Acute Esophagitis Leading to Acute Esophageal Epithelium Lesions.

Authors - Katarzyna Magierowska
ratWB

PLoS One

Carbon Monoxide (CO) Released from Tricarbonyldichlororuthenium (II) Dimer (CORM-2) in Gastroprotection against Experimental Ethanol-Induced Gastric Damage.

Authors - Katarzyna Magierowska