• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FBXL16 Polyclonal antibody

FBXL16 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25950-1-AP

Synonyms

C16orf22, c380A1.1, F box/LRR repeat protein 16, Fbl16, FBXL16

Validation Data Gallery

Applications:
  • WB analysis of HeLa using 25950-1-AP

    WB analysis of HeLa using 25950-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 25950-1-AP (FBXL16 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human brain using 25950-1-AP

    IHC staining of human brain using 25950-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25950-1-AP (FBXL16 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human brain using 25950-1-AP

    IHC staining of human brain using 25950-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 25950-1-AP (FBXL16 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 1 publications below

Product Information

The immunogen of 25950-1-AP is FBXL16 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXL16 fusion protein Ag22827 相同性解析による交差性が予測される生物種
Full Name F-box and leucine-rich repeat protein 16
Calculated molecular weight 479 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC036680
Gene symbol FBXL16
Gene ID (NCBI) 146330
RRIDAB_2880307
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FBXL16 antibody 25950-1-APDownload protocol
IHC protocol for FBXL16 antibody 25950-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Nat Chem Biol

LATS2 condensates organize signalosomes for Hippo pathway signal transduction

Authors - Min Qin
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Nazia (Verified Customer) (01-06-2022)

Package arrived very quickly and staff were very helpful. Product worked just as expected. Great customer service. I will be recommending ProteinTech to colleagues in my lab.

  • Applications: Western Blot