• Featured Product
  • KD/KO Validated

FABP3 Polyclonal antibody

FABP3 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10676-1-AP

Synonyms

FABP11, FABP3, Fatty acid binding protein 3, H FABP, HFABP, M FABP, MDGI, O FABP



Tested Applications

Positive WB detected inmouse heart tissue, rat testis tissue, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue
Positive IHC detected inmouse heart tissue, human skin tissue, human lung tissue, human ovary tissue, human breast cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse brain tissue
Planning an IHC experiment? We recommend our IHCeasy FABP3 Ready-To-Use IHC Kit. FABP3 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

10676-1-AP targets FABP3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, rat, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FABP3 fusion protein Ag1069 相同性解析による交差性が予測される生物種
Full Name fatty acid binding protein 3, muscle and heart (mammary-derived growth inhibitor)
Calculated molecular weight 15 kDa
Observed molecular weight 15 kDa
GenBank accession numberBC007021
Gene symbol FABP3
Gene ID (NCBI) 2170
RRIDAB_2102309
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FABP3 (fatty-acid-binding protein 3), also known as heart-type FABP or mammary-derived growth inhibitor (MDGI), is a small 15-kDa cytoplasmic protein transporting fatty acids and other lipophilic substances from the cytoplasm to the nucleus. It is most ubiquitously expressed in heart and skeletal muscle.

Protocols

Product Specific Protocols
WB protocol for FABP3 antibody 10676-1-APDownload protocol
IHC protocol for FABP3 antibody 10676-1-APDownload protocol
IF protocol for FABP3 antibody 10676-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

ACS Nano

Nanointegrative In Situ Reprogramming of Tumor-Intrinsic Lipid Droplet Biogenesis for Low-Dose Radiation-Activated Ferroptosis Immunotherapy

Authors - Qiqi Zhang
mouseWB

Biomaterials

MMP-12 siRNA improves the homeostasis of the small intestine and metabolic dysfunction in high-fat diet feeding-induced obese mice.

Authors - Mingming Song
mouseWB

J Neurosci

FABP3 in the Anterior Cingulate Cortex Modulates the Methylation Status of the Glutamic Acid Decarboxylase67 Promoter Region.

Authors - Yui Yamamoto
mouseWB

Int J Mol Sci

Amelioration of Nicotine-Induced Conditioned Place Preference Behaviors in Mice by an FABP3 Inhibitor

Authors - Wenbin Jia
mouseWB

J Cell Mol Med

PMID: 21251210

Authors - Xi Lei L
mouseWB,IF

J Biol Chem

FABP3 promotes αSyn oligomerization associated with MPTP-induced neurotoxicity.

Authors - Norifumi Shioda
  • KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sampath Kumar (Verified Customer) (02-26-2024)

The FABP3 antibody worked well at 1:1000 dilution on rodent skeletal muscle tissue homogenates (6ug total protein), producing bands at 15kDa. However, homogenates from Avian pectoralis muscle homogenates (6ug total protein) produced barely visible bands.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Avian and rodent skeletal muscle
FABP3 Antibody Western Blot validation (1:1000 dilution) in Avian and rodent skeletal muscle (Cat no:10676-1-AP)