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KD/KO Validated

DGKA Polyclonal antibody

Name: DGKA Polyclonal antibody
Brand: Proteintech
SKU: 11547-1-AP

DGKA Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11547-1-AP

Synonyms

80 kDa diacylglycerol kinase, DAG kinase alpha, DAGK, DAGK1, DGK alpha, DGKA, Diacylglycerol kinase alpha, Diglyceride kinase alpha

Validation Data Gallery

Applications:

AllWBIPIHCIFFC

WB analysis using 11547-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11547-1-AP (DGKA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 11547-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11547-1-AP (DGKA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 11547-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11547-1-AP (DGKA antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis of mouse cerebellum using 11547-1-AP
mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 11547-1-AP (DGKA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HEK-293 using 11547-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 11547-1-AP (DGKA Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of human cerebellum using 11547-1-AP
human cerebellum tissue were subjected to SDS PAGE followed by western blot with 11547-1-AP (DGKA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
IP experiment of Jurkat using 11547-1-AP
IP result of anti-DGKA (IP:11547-1-AP, 3ug; Detection:11547-1-AP 1:1000) with Jurkat cells lysate 1500ug.
IHC staining of human gliomas using 11547-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11547-1-AP (DGKA antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human gliomas using 11547-1-AP
Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 11547-1-AP (DGKA antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human brain using 11547-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 11547-1-AP (DGKA antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human brain using 11547-1-AP
Immunohistochemical analysis of paraffin-embedded human brain using 11547-1-AP (DGKA antibody) at dilution of 1:100 (under 40x lens).
IF Staining of Jurkat using 11547-1-AP
Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using DGKA antibody (11547-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).
IF Staining of Jurkat using 11547-1-AP
Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using DGKA antibody (11547-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 11547-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human DGKA (11547-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 11547-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Jurkat cells, HeLa cells, MOLT-4 cells, human cerebellum tissue, HEK-293 cells, mouse cerebellum tissue
Positive IP detected in	Jurkat cells
Positive IHC detected in	human gliomas tissue, human brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF detected in	Jurkat cells
Positive FC detected in	HeLa cells

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:12000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)	IF : 1:50-1:500
Flow Cytometry (FC)	FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Published Applications

KD/KO	See 7 publications below
WB	See 15 publications below
IHC	See 5 publications below
IF	See 1 publications below
IP	See 1 publications below

Product Information

11547-1-AP targets DGKA in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	DGKA fusion protein Ag2075 相同性解析による交差性が予測される生物種
Full Name	diacylglycerol kinase, alpha 80kDa
Calculated molecular weight	735 aa, 83 kDa
Observed molecular weight	83 kDa
GenBank accession number	BC023523
Gene symbol	DGKA
Gene ID (NCBI)	1606
RRID	AB_2245857
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

DGKA(Diacylglycerol kinase alpha) is also named as DAGK, DAGK1 and belongs to the eukaryotic diacylglycerol kinase family,which is suggested to attenuate diacylglycerol-induced cell responses through the phosphorylation of this second messenger to phosphatidic acid.DGKA regulates the secretion of lethal exosomes bearing Fas ligand during activation-induced cell death of T lymphocytes.

Protocols

Product Specific Protocols
WB protocol for DGKA antibody 11547-1-AP	Download protocol
IHC protocol for DGKA antibody 11547-1-AP	Download protocol
IF protocol for DGKA antibody 11547-1-AP	Download protocol
IP protocol for DGKA antibody 11547-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
		Nat Commun Mutant p53s generate pro-invasive niches by influencing exosome podocalyxin levels. Authors - David Novo View Article
human	WB	Nat Chem Biol Reprogramming fatty acyl specificity of lipid kinases via C1 domain engineering. Authors - Timothy B Ware KD Validated View Article
human	WB	Nat Commun Epigenetic regulation of diacylglycerol kinase alpha promotes radiation-induced fibrosis. Authors - Christoph Weigel View Article
human	WB	Cancer Discov Diacylglycerol Kinase α Is a Critical Signaling Node and Novel Therapeutic Target in Glioblastoma and Other Cancers. Authors - Dominguez Charli L CL KD Validated View Article
human	WB,IHC	Clin Cancer Res DGKA Provides Platinum Resistance in Ovarian Cancer Through Activation of c-JUN-WEE1 Signaling. Authors - Jie Li KD Validated View Article
mouse	WB	J Immunother Cancer Diacylglycerol kinase ζ limits IL-2-dependent control of PD-1 expression in tumor-infiltrating T lymphocytes. Authors - Javier Arranz-Nicolás View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

DGKA Polyclonal antibody

DGKA Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Validation Data Gallery

WB analysis using 11547-1-AP

WB analysis using 11547-1-AP

WB analysis using 11547-1-AP

WB analysis of mouse cerebellum using 11547-1-AP

WB analysis of HEK-293 using 11547-1-AP

WB analysis of human cerebellum using 11547-1-AP

IP experiment of Jurkat using 11547-1-AP

IHC staining of human gliomas using 11547-1-AP

IHC staining of human gliomas using 11547-1-AP

IHC staining of human brain using 11547-1-AP

IHC staining of human brain using 11547-1-AP

IF Staining of Jurkat using 11547-1-AP

IF Staining of Jurkat using 11547-1-AP

FC experiment of HeLa using 11547-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Mutant p53s generate pro-invasive niches by influencing exosome podocalyxin levels.

Reprogramming fatty acyl specificity of lipid kinases via C1 domain engineering.

Epigenetic regulation of diacylglycerol kinase alpha promotes radiation-induced fibrosis.

Diacylglycerol Kinase α Is a Critical Signaling Node and Novel Therapeutic Target in Glioblastoma and Other Cancers.

DGKA Provides Platinum Resistance in Ovarian Cancer Through Activation of c-JUN-WEE1 Signaling.

Diacylglycerol kinase ζ limits IL-2-dependent control of PD-1 expression in tumor-infiltrating T lymphocytes.