• Featured Product
  • KD/KO Validated

CRBN Polyclonal antibody

CRBN Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11435-1-AP

Synonyms

cereblon, CRBN, MRT2A, Protein cereblon



Tested Applications

Positive WB detected inmouse heart tissue, COLO 320 cells, human brain tissue, human heart tissue, A375 cells, mouse liver tissue
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCOLO 320 cells, A375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

11435-1-AP targets CRBN in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CRBN fusion protein Ag1951 相同性解析による交差性が予測される生物種
Full Name cereblon
Calculated molecular weight 442 aa, 51 kDa
Observed molecular weight 51-55 kDa
GenBank accession numberBC017419
Gene symbol CRBN
Gene ID (NCBI) 51185
RRIDAB_2085739
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CRBN plays an important role in assembly and surface expression of functional BK(Ca) channels. The mutation of human cereblon gene (CRBN) is related with mild mental retardation, and causes ARNSID (nonsyndromal intellectual disability) by distubing the developmental regulation of BK(Ca) in brain regions that are critical for memory and learning. CRBN is also identified as important thalidomide-binding protein for limb outgrowth in zebrafish and chicks. It can be detected a band above 51 kDa (maybe 55 kDa) in HT22 cells(PMID: 21232561).

Protocols

Product Specific Protocols
WB protocol for CRBN antibody 11435-1-APDownload protocol
IHC protocol for CRBN antibody 11435-1-APDownload protocol
IF protocol for CRBN antibody 11435-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Science

DRUG DEVELOPMENT. Phthalimide conjugation as a strategy for in vivo target protein degradation.

Authors - Georg E Winter
  • KO Validated
humanWB

Mol Cell

BET Bromodomain Proteins Function as Master Transcription Elongation Factors Independent of CDK9 Recruitment.

Authors - Georg E Winter
  • KO Validated
humanWB

Nat Commun

Homo-PROTACs: bivalent small-molecule dimerizers of the VHL E3 ubiquitin ligase to induce self-degradation.

Authors - Chiara Maniaci
humanWB

Cell Rep

The Drug Vehicle and Solvent N-Methylpyrrolidone Is an Immunomodulator and Antimyeloma Compound.

Authors - Jake Shortt
humanWB

Elife

Targeted degradation of BRD9 reverses oncogenic gene expression in synovial sarcoma.

Authors - Gerard L Brien
mouseWB

FASEB J

AMPK is down-regulated by the CRL4A-CRBN axis through the polyubiquitination of AMPKα isoforms.

Authors - Eunju Kwon
  • KO Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Elisa (Verified Customer) (02-07-2022)

RPE1 cells were fixed in cold methanol for 10' at -20C . Cells were then rehydrated with PBS for 5'. Membrane permeabilization was then performed with 0.1% Triton + 0.1% Tween +0.01%SDS in PBS for 5'. Cells were finally incubated with blocking buffer (5% BSA+ 0.1% Tween in PBS) for 30' at RT. Primary antibody was diluted in blocking buffer 1:200 and incubated for 1h at room temperature. Alexa-555-Anti-rabbit was used as secondary antibody (1:600 dilution) (1h at room temperature).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Human retinal pigment epithelial-1 (RPE-1)
CRBN Antibody Immunofluorescence validation (1:200 dilution) in Human retinal pigment epithelial-1 (RPE-1) (Cat no:11435-1-AP)
FH

LUNFENG (Verified Customer) (01-27-2020)

GOOD

  • Applications: Immunofluorescence,
  • Cell Tissue Type: HEART
FH

Jing (Verified Customer) (01-27-2020)

Tested in human hiPSC-derived cardiomyocytes, it showed very clean single band around 60 kd.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: cardiomyocytes
FH

Yan (Verified Customer) (01-03-2020)

We tested CBRN antibody in mouse heart and brain tissue extracts. CRBN antibody have specificity to recognize CRBN by western blot which was confirmed by knockout mice.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse brain and heart tissue
FH

Jie (Verified Customer) (12-27-2019)

works specifically with one clear band at the right size

  • Applications: Western Blot, Immunohistochemistry, Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: cardiomtocytes
FH

Sarah (Verified Customer) (07-04-2019)

Total cell lysate (15 ug) was resolved on a 4-12% Bis-Tris gel and transferred to nitrocellulose membrane. Membrane was incubated in blocking buffer (5% milk/0.1% Tween-20) for 1h. Membrane was incubated with anti-CRBN in blocking buffer (1:1000) at 4C overnight. After washing, membrane was incubated in anti-rabbit-HRP in blocking bufffer (1:3000) for 1h at room temperature. Protein was detected using ECL reagent and imaged on a chemiluminescence detection system.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: U251/Jurkat
CRBN Antibody Western Blot, validation (1:1000 dilution) in U251/Jurkat (Cat no:11435-1-AP)