Collagen Type IV Polyclonal antibody

Collagen Type IV Polyclonal Antibody for IF,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Cat no : 19674-1-AP

Synonyms

CA44, COL4A4, Collagen alpha 4(IV) chain, Collagen Type IV, collagen, type IV, alpha 4



Tested Applications

Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

19674-1-AP targets Collagen Type IV in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name collagen, type IV, alpha 4
Calculated molecular weight 164 kDa
GenBank accession numberNM_000092
Gene symbol COL4A4
Gene ID (NCBI) 1286
RRIDAB_2878595
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

COL4A4 belongs to the type IV collagen family. COL4A4 is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen. Defects in COL4A4 are a cause of Alport syndrome autosomal recessive (APSAR). Defects in COL4A4 are a cause of benign familial hematuria (BFH). This antibody is specific to COL4A4.

Protocols

Product Specific Protocols
IF protocol for Collagen Type IV antibody 19674-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

J Ethnopharmacol

In silico prediction and experimental validation to reveal the protective mechanism of Puerarin against excessive extracellular matrix accumulation through inhibiting ferroptosis in diabetic nephropathy

Authors - Biyu Hou
ratWB

Eur J Pharmacol

Sirt1 activation prevents anti-Thy 1.1 mesangial proliferative glomerulonephritis in the rat through the Nrf2/ARE pathway.

Authors - Kaipeng Huang
ratIF

J Chem Neuroanat

Berberine protects against chronic cerebral hypoperfusion-induced cognitive impairment and hippocampal damage via regulation of the ERK/Nrf2 pathway.

Authors - Ningqun Wang