CD7 Monoclonal antibody
CD7 Monoclonal Antibody for IF, IHC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse
Applications
IHC, IF, FC, ELISA
Conjugate
Unconjugated
CloneNo.
1G10D8
Cat no : 60209-1-Ig
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive IHC detected in | human appendicitis tissue, human tonsillitis tissue, mouse thymus tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human tonsillitis tissue |
Planning an IHC experiment? We recommend our IHCeasy CD7 Ready-To-Use IHC Kit. CD7 primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:1600-1:6400 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Sample-dependent, check data in validation data gallery | |
Published Applications
| IF | See 1 publications below |
| FC | See 1 publications below |
Product Information
60209-1-Ig targets CD7 in IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | CD7 fusion protein Ag1805 相同性解析による交差性が予測される生物種 |
| Full Name | CD7 molecule |
| Calculated molecular weight | 240 aa, 25 kDa |
| GenBank accession number | BC009293 |
| Gene symbol | CD7 |
| Gene ID (NCBI) | 924 |
| RRID | AB_10953398 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
CD7 is a 40 kD type I transmembrane glycoprotein expressed on thymocytes and mature T cells. It is the most reliable clinical marker of T-cell acute lymphocytic leukemia.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CD7 antibody 60209-1-Ig | Download protocol |
| IHC protocol for CD7 antibody 60209-1-Ig | Download protocol |
| IF protocol for CD7 antibody 60209-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cell Metab Bone marrow immune cells respond to fluctuating nutritional stress to constrain weight regain | ||
Small In Vivo mRNA CAR T Cell Engineering via Targeted Ionizable Lipid Nanoparticles with Extrahepatic Tropism |
