CD23 Monoclonal antibody

CD23 Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3H6B8

Cat no : 60208-1-Ig

Synonyms

BLAST 2, CD23, CD23A, CLEC4J, Fc epsilon RII, FCE2, FCER2, IGEBF, Lymphocyte IgE receptor



Tested Applications

Positive WB detected inDC2.4 cells, Raji cells, Ramos cells, Daudi cells
Positive IHC detected inhuman tonsillitis tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inhuman tonsillitis tissue
Planning an IHC experiment? We recommend our IHCeasy CD23 Ready-To-Use IHC Kit. CD23 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 60208-1-Ig is CD23 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CD23 fusion protein Ag0425 相同性解析による交差性が予測される生物種
Full Name Fc fragment of IgE, low affinity II, receptor for (CD23)
Calculated molecular weight 321 aa, 36 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC064417
Gene symbol FCER2
Gene ID (NCBI) 2208
RRIDAB_10949195
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD23, also known as low affinity immunoglobulin epsilon Fc receptor, is a transmembrane glycoprotein present on a subpopulation of B lymphocytes in germinal centres, EBV-transformed B-lymphoblastoid cell lines, follicular dendritic cells, and a subpopulation of peripheral blood cells. CD23 has essential roles in the regulation of IgE production and in the differentiation of B-cells.

Protocols

Product Specific Protocols
WB protocol for CD23 antibody 60208-1-IgDownload protocol
IHC protocol for CD23 antibody 60208-1-IgDownload protocol
IF protocol for CD23 antibody 60208-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC,IF

Clin Transl Med

Single-cell transcriptome sequencing of B-cell heterogeneity and tertiary lymphoid structure predicts breast cancer prognosis and neoadjuvant therapy efficacy

Authors - Qing Wang
humanIF

Sci Signal

Short stature and combined immunodeficiency associated with mutations in RGS10.

Authors - Ivan K Chinn
mouseWB,IF

Biol Pharm Bull

Indomethacin, a Non-steroidal Anti-inflammatory Drug, Induces Skin Dryness via PPARγ in Mice.

Authors - Kiyoko Maruyama
humanIHC

BMC Musculoskelet Disord

Anti-signal recognition particle positive necrotizing myopathy-sjogren's syndrome overlap syndrome: a descriptive study on clinical and myopathology features

Authors - Li Xu