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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD2 Polyclonal antibody

CD2 Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 23973-1-AP

Synonyms

CD2, CD2 molecule, Erythrocyte receptor, LFA 2, LFA 3 receptor, Rosette receptor, SRBC, T cell surface antigen CD2, T11

Validation Data Gallery

Applications:
  • WB analysis of Jurkat using 23973-1-AP

    WB analysis of Jurkat using 23973-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 23973-1-AP (CD2 Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 23973-1-AP

    IHC staining of human tonsillitis using 23973-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 23973-1-AP (CD2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

  • IHC staining of human tonsillitis using 23973-1-AP

    IHC staining of human tonsillitis using 23973-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 23973-1-AP (CD2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieved with Tris-EDTA buffer, pH9.0.

  • IF Staining of Jurkat using 23973-1-AP

    IF Staining of Jurkat using 23973-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using 23973-1-AP (CD2 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of Jurkat using 23973-1-AP

    FC experiment of Jurkat using 23973-1-AP

    1X10^6 Jurkat cells were stained with 0.20 ug Anti-Human CD2 (23973-1-AP) (red) or 0.20 ug isotype control antibody (blue) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000. Cells were fixed with 90% MeOH.

Tested Applications

Positive WB detected inJurkat cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inJurkat cells
Positive FC detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23973-1-AP is CD2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD2 fusion protein Ag21122 相同性解析による交差性が予測される生物種
Full Name CD2 molecule
Calculated molecular weight 351 aa, 39 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC033583
Gene symbol CD2
Gene ID (NCBI) 914
RRIDAB_2879384
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD2 is a cell surface glycoprotein present on a majority of thymocytes, all mature T cells and subset of NK cells but not on B lymphocytes. It is a pan T-cell marker. CD2 interacts with lymphocyte function-associated antigen (LFA-3/CD58) and CD48/BCM1 to mediate adhesion between T-cells and other cell types. CD2 is implicated in the triggering of T-cells, the cytoplasmic domain is implicated in the signaling function.

Protocols

Product Specific Protocols
WB protocol for CD2 antibody 23973-1-APDownload protocol
IHC protocol for CD2 antibody 23973-1-APDownload protocol
IF protocol for CD2 antibody 23973-1-APDownload protocol
FC protocol for CD2 antibody 23973-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols