CD14 Polyclonal antibody

CD14 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (2)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 17000-1-AP

Synonyms

CD14, CD14 molecule



Tested Applications

Positive WB detected inHL-60 cells, RAW 264.7 cells, human spleen tissue
Positive IHC detected inhuman hepatocirrhosis tissue, human appendicitis tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inRAW 264.7 cells
Positive FC detected inRAW 264.7 cells
Planning an IHC experiment? We recommend our IHCeasy CD14 Ready-To-Use IHC Kit. CD14 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

17000-1-AP targets CD14 in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human, RAT, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen CD14 fusion protein Ag10693 相同性解析による交差性が予測される生物種
Full Name CD14 molecule
Calculated molecular weight 375 aa, 40 kDa
Observed molecular weight 50-55 kDa
GenBank accession numberBC010507
Gene symbol CD14
Gene ID (NCBI) 929
RRIDAB_2074048
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD14 is a 50-55 kDa glycosylphosphatidylinositol-anchored glycoprotein preferentially expressed on monocytes and macrophages, and at lower levels on granulocytes (PMID: 3385210; 2462937; 7685797). CD14 can also exist as a soluble protein. CD14 acts as a co-receptor for bacterial liposaccharides (LPS) (PMID: 1698311). It plays a major role in the inflammatory response of monocytes to LPS.

Protocols

Product Specific Protocols
WB protocol for CD14 antibody 17000-1-APDownload protocol
IHC protocol for CD14 antibody 17000-1-APDownload protocol
IF protocol for CD14 antibody 17000-1-APDownload protocol
FC protocol for CD14 antibody 17000-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Br J Sports Med

Chronic inflammation is a feature of Achilles tendinopathy and rupture.

Authors - Stephanie Georgina Dakin
mouseWB

Sci Immunol

Clostridium septicum α-toxin activates the NLRP3 inflammasome by engaging GPI-anchored proteins.

Authors - Weidong Jing
human,mouse,ratFC

Nat Commun

HIF1α inhibition facilitates Leflunomide-AHR-CRP signaling to attenuate bone erosion in CRP-aberrant rheumatoid arthritis.

Authors - Chao Liang
humanIF

EBioMedicine

Patient-derived melanoma organoid models facilitate the assessment of immunotherapies

Authors - Lingling Ou
mouseIF

Biomaterials

Construction of functional magnetic scaffold with temperature control switch for long-distance vascular injury

Authors - Xin Liu
ratIF

Biomaterials

Cerebro- and renoprotective activities through platelet-derived biomaterials against cerebrorenal syndrome in rat model.

Authors - Hon-Kan Yip

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Emma (Verified Customer) (11-29-2021)

Works well by IF on FFPE tissue @ 1:200 with a Tris-EDTA antigen retrieval.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Prostate