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CAPZA1 Monoclonal antibody

CAPZA1 Monoclonal Antibody for IF, WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

10H1F9

Cat no : 66066-1-Ig

Synonyms

CAPPA1, CAPZ, CapZ alpha 1, CAPZA1, CAZ1

Validation Data Gallery

Applications:
  • WB analysis of HEK-293 using 66066-1-Ig

    WB analysis of HEK-293 using 66066-1-Ig

    WB result of CAPZA1 antibody (66066-1-Ig, 1:8000) with si-Control and si-CAPZA1 transfected HEK293 cells.

  • WB analysis using 66066-1-Ig

    WB analysis using 66066-1-Ig

    Western blot analysis of CAPZA1 in various cell lines using Proteintech antibody 66066-1-Ig at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • WB analysis of Jurkat using 66066-1-Ig

    WB analysis of Jurkat using 66066-1-Ig

    Jurkat cells were subjected to SDS PAGE followed by western blot with 66066-1-Ig (CAPZA1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 66066-1-Ig

    IF Staining of HeLa using 66066-1-Ig

    Immunofluorescent analysis of Hela cells using 66066-1-Ig (CAPZA1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

  • IF Staining of MCF-7 using 66066-1-Ig

    IF Staining of MCF-7 using 66066-1-Ig

    Immunofluorescent analysis of () fixed MCF-7 cells using 66066-1-Ig (CAPZA1 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

Tested Applications

Positive WB detected inHEK-293 cells, Jurkat cells, ROS1728 cells, PC-12 cells, RAW 264.7 cells, NIH/3T3 cells
Positive IF detected inHela cells, MCF-7 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Published Applications

IFSee 1 publications below

Product Information

66066-1-Ig targets CAPZA1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen CAPZA1 fusion protein Ag18841 相同性解析による交差性が予測される生物種
Full Name capping protein (actin filament) muscle Z-line, alpha 1
Calculated molecular weight 286 aa, 33 kDa
Observed molecular weight 37 kDa
GenBank accession numberBC000144
Gene symbol CAPZA1
Gene ID (NCBI) 829
RRIDAB_11042588
Conjugate Unconjugated
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CAPZA1 (F-actin-capping protein subunit alpha-1) is a member of the F-actin capping protein alpha subunit family and regulates actin filament growth, stability and disassembly. Differential expression of CAPZA1 has been found in gastric cancer and melanoma, indicating its possible role in tumorigenesis. (21566537, 23545944)

Protocols

Product Specific Protocols
WB protocol for CAPZA1 antibody 66066-1-IgDownload protocol
IF protocol for CAPZA1 antibody 66066-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Cancers (Basel)

Critical Role for Cold Shock Protein YB-1 in Cytokinesis.

Authors - Sunali Mehta
View Article