Apolipoprotein AI Polyclonal antibody

Apolipoprotein AI Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 14427-1-AP

Synonyms

Apo AI, ApoA I, APOA1, apolipoprotein A I, Apolipoprotein A1



Tested Applications

Positive WB detected inhuman blood tissue, human brain tissue, human plasma, human ileum tissue
Positive IHC detected inhuman liver cancer tissue, human liver tissue, human lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells, HeLa cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

14427-1-AP targets Apolipoprotein AI in WB, IP, IHC, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Apolipoprotein AI fusion protein Ag5793 相同性解析による交差性が予測される生物種
Full Name apolipoprotein A-I
Calculated molecular weight 31 kDa
Observed molecular weight 26-30 kDa
GenBank accession numberBC005380
Gene symbol APOA1
Gene ID (NCBI) 335
RRIDAB_2056524
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ApoA1 is a major protein component of high density lipoproteins (HDL) which is associated with reversed cholesterol transport, lipid/cholesterol binding, lecithin/cholesterol acyltransferase (LCAT) activation and specific receptors binding. It is synthesized in the liver and small intestine. Defects of ApoA1 cause low HDL level and systemic non-neuropathic amyloidosis. Serum concentration of ApoA1 is inversely related to the risk of developing atherosclerosis. This antibody was generated against the C-terminal region of human ApoA1.

Protocols

Product Specific Protocols
WB protocol for Apolipoprotein AI antibody 14427-1-APDownload protocol
IHC protocol for Apolipoprotein AI antibody 14427-1-APDownload protocol
IF protocol for Apolipoprotein AI antibody 14427-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
IP

Science

Enterically derived high-density lipoprotein restrains liver injury through the portal vein.

Authors - Yong-Hyun Han
mouseWB,IP

Neuron

Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.

Authors - Xiaohui Li
humanIHC

Br J Cancer

A new panel of pancreatic cancer biomarkers discovered using a mass spectrometry-based pipeline.

Authors - Xiaohui Liu
humanWB

Theranostics

Exosome-mediated delivery of inflammation-responsive Il-10 mRNA for controlled atherosclerosis treatment

Authors - Te Bu

Br J Pharmacol

A novel apoA-I mimetic peptide suppresses atherosclerosis by promoting physiological HDL function in apoE-/- mice.

Authors - Sanhu Gou
humanIHC

Am J Obstet Gynecol

Trophoblast apoptosis from pregnancies complicated by fetal growth restriction is associated with enhanced p53 expression.

Authors - Roni Levy

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Alexandre (Verified Customer) (02-28-2019)

Detection of both human and mouse APOA1 protein by westernblotImmunoprecipitation of both human and mouse APOA1

  • Applications: Western Blot, Immunoprecipitation,
  • Primary Antibody Dilution: WB 1/1000
  • Cell Tissue Type: HepG2 / Mouse Liver
Apolipoprotein AI Antibody Western Blot,Immunoprecipitation, validation (WB 1/1000 dilution) in HepG2 / Mouse Liver (Cat no:14427-1-AP)